| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 21952 | Journal of Bioscience and Bioengineering | 2009 | 4 Pages |
Abstract
Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20–55 °C and 2–12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Shusuke Takahashi, Toshiki Furuya, Yoshitaka Ishii, Kuniki Kino, Kohtaro Kirimura,