Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2199769 | Molecular and Cellular Probes | 2013 | 4 Pages |
Abstract
In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the ‘zoonotic’ assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia.
Keywords
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Authors
Yvonne A.L. Lim, Mohammed A.K. Mahdy, Tiong K. Tan, Xiang T. Goh, Aaron R. Jex, Matthew J. Nolan, Reuben S.K. Sharma, Robin B. Gasser,