The application of a polymerase chain reaction (PCR)-based capillary electrophoretic technique provides detailed insights into Eimeria populations in intensive poultry establishments

Intestinal coccidiosis, caused by one or multiple species of Eimeria (Protozoa: Apicomplexa), is one of the most important infectious diseases affecting chickens. In this study, we used a polymerase chain reaction (PCR)-based capillary electrophoresis (CE) approach to conduct an epidemiological survey of Eimeria species in seven Australian broiler flocks, varying in age from 18 to 42 days. We confirmed that all seven recognized Eimeria species of poultry were present. Eimeria acervulina and E. maxima were the most common, followed by E. mitis (i.e., 89%, 87% and 64% of chickens, respectively). E. praecox was present in 44% of birds, whereas E. brunetti and E. tenella were uncommon, being found in 36% and 26%, respectively. E. necatrix was rarely detected (10%). Even the least common species were present in more than 70% of sheds. The prevalence of individual species was higher in older than in younger chickens. Most of the chickens sampled were simultaneously infected with multiple Eimeria species (mean=3.6). The number of Eimeria oocysts excreted per gram of faeces reached a peak at 36 days of age, before declining to a considerably lower level by 42 days. As anticoccidial drugs were permanently withdrawn at 36 days, the decreasing Eimeria oocyst excretion rates indicated the development of protective immunity in the chickens. The present study showed that even healthy chickens usually harbour numerous species of Eimeria. The CE technique proved to be a time and cost-effective means of investigating the epidemiology of Eimeria in commercial establishments.