| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2200099 | Molecular and Cellular Probes | 2007 | 9 Pages |
Abstract
We developed a multiplex PCR for amplification of ten genes involved in resistance to ciprofloxacin, doxycycline, rifampin, and vancomycin in Bacillus anthracis and closely related Bacillus cereus. Enzymatic labelling of PCR products followed by hybridization to oligonucleotide probes on a DNA microarray enabled simultaneous detection of resistance genes tetK, tetL, tetM, tetO, vanA, and vanB and resistance-mediating point mutations in genes gyrA, gyrB, parC, and rpoB. The presented assay allows detection of clinically relevant antibiotic resistance determinants within 4 h and can be used as a time-saving tool supporting conventional culture-based diagnostics.
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Authors
Markus H. Antwerpen, Michaela Schellhase, Eva Ehrentreich-Förster, Frank Bier, Wolfgang Witte, Ulrich Nübel,