Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2200170 | Molecular and Cellular Probes | 2006 | 5 Pages |
Abstract
Plasmodium falciparum drug resistance is a major factor for the death toll of malaria. Resistance has been associated with specific single nucleotide polymorphisms (SNPs) in the parasite genes pfmdr1 (N86Y) and pfcrt (K76T) associated with quionoline antimalarial resistance, and pfdhfr (N51I, C59R, S108N) correlated with resistance of the antifolate combination sulfadoxine–pyrimethamine. These SNPs constitute the basis for the surveillance of drug resistance through high sensitive molecular methods in malaria endemic countries. In this work, we developed a multiplex PCR–RFLP protocols for the diagnosis of these molecular markers, leading to significant decreases in reagent costs, time, number of manipulations and hence human resources.
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Authors
Maria Isabel Veiga, Pedro Eduardo Ferreira, Anders Björkman, José Pedro Gil,