Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
22042 | Journal of Bioscience and Bioengineering | 2008 | 5 Pages |
Abstract
Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2–0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Kazuo Yamagishi, Minetaka Sugiyama, Yoshinobu Kaneko, Masafumi Nishizawa, Satoshi Harashima,