Article ID Journal Published Year Pages File Type
22132 Journal of Bioscience and Bioengineering 2007 4 Pages PDF
Abstract

A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/μg of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.

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Physical Sciences and Engineering Chemical Engineering Bioengineering
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