Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
22132 | Journal of Bioscience and Bioengineering | 2007 | 4 Pages |
Abstract
A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/μg of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Tomotake Morita, Hiroshi Habe, Tokuma Fukuoka, Tomohiro Imura, Dai Kitamoto,