Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
22159 | Journal of Bioscience and Bioengineering | 2007 | 4 Pages |
Abstract
A gene-tagging mutagenesis method by random integration of linear DNA fragments was developed and used in the methylotrophic yeast Candida boidinii to isolate mutants defective in methanol-inducible gene expression. A large number of mutants were obtained, indicating that this method is a powerful tool for random mutagenesis in C. boidinii.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Yu Sasano, Hiroya Yurimoto, Yasuyoshi Sakai,