Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
22173 | Journal of Bioscience and Bioengineering | 2006 | 4 Pages |
Abstract
The cpsIaJ gene of Streptococcus agalactiae type Ia codes for β-1,4-galactosyltransferase. In this study, the functional expression of His-tagged CpsIaJ in a baculovirus expression system was performed, because the efficient functional expression of this enzyme in Escherichia coli had been unsuccessful. Using a partially purified enzyme preparation, we found that the enzyme had a restricted substrate specificity and that the entire structure of the substrate GlcNAcβ1-3Galβ1-4Glc was required for the activity. Furthermore, mutations in a conserved DXD motif caused the loss of the enzyme's activity.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Yohei Kataoka, Shingo Ozeki, Katsuhide Miyake, Shinji Iijima,