Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
22404 | Journal of Bioscience and Bioengineering | 2008 | 4 Pages |
Abstract
Recombinant Aspergillus oryzae expressing a dye-decolorizing peroxidase gene (dyp) was cultivated for repeated-batch production of recombinant dye-decolorizing peroxidase (rDyP) using maltose as a carbon source. High-level rDyP activity in limitation of carbon and nitrogen sources was maintained stably for 26 cycles of repeated 1-d batches of A. oryzae pellets without any additional pH control. Cultures maintained at 4°C for 20 d resumed rDyP production following a single day of incubation. One liter filtrated crude rDyP containing 4600 U rDyP decolorized 5.07 g RBBR at the apparent decolorization rate of 17.7 mg l−1 min−1.
Related Topics
Physical Sciences and Engineering
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Authors
Mozaffar Shakeri, Yasushi Sugano, Makoto Shoda,