Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
22454 | Journal of Bioscience and Bioengineering | 2008 | 4 Pages |
Abstract
The efficiency of bioactive-beads-mediated plant transformation was improved using DNA-lipofectin complex as the entrapped genetic material instead of naked DNA used in the conventional method. In the improved method, beads aggregated and formed clusters around the protoplasts resulting in a 4-fold higher transformation efficiency than that by the conventional method.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Tomoko Murakawa, Shin'ichiro Kajiyama, Tomohiko Ikeuchi, Shigeki Kawakami, Kiichi Fukui,