Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
22467 | Journal of Bioscience and Bioengineering | 2007 | 4 Pages |
Abstract
We found 11 genes (FAO1-11) encoding putative oxidoreductases in the Aspergillus oryzae genome, which are similar to fungal fructosyl-amino acid oxidases. The cDNAs corresponding to the genes were cloned and expressed in Escherichia coli. rFao2 had fructosyl-amino acid oxidase activity, whereas rFao1 did not show any enzyme activity, even though the deduced amino acid sequence of Fao1 is identical to that of one of the fructosyl-amino acid oxidase isozymes from Aspergillus oryzae. rFao7 and rFao8 showed oxidase activity toward sarcosine, L-pipecolate, and L-proline. rFao10 was active toward only sarcosine, of the substrates tested. The functions of the other proteins were also predicted from a phylogenetic analysis.
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Authors
Nobuyuki Yoshida, Shin-Ichi Akazawa, Tetsuya Karino, Hiroki Ishida, Yoji Hata, Tohoru Katsuragi, Yoshiki Tani, Hiroshi Takagi,