Novel Technique for Fabricating Double-Layered Tubular Constructs Consisting of Two Vascular Cell Types in Collagen Gels Used as Templates for Three-Dimensional Tissues

Double-layered tubular constructs consisting of two vascular cell types in collagen gels were fabricated using a previously developed technique [Takei et al., Biotechnol. Bioeng., 95, 1–7 (2006)]. Histological examination suggested that shear stress (4.0 dyn/cm2) on the luminal surface of the constructs induced morphological changes in their walls.