| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 22489 | Journal of Bioscience and Bioengineering | 2007 | 4 Pages |
Abstract
We developed an enzyme-linked immunosorbent assay for an endocrine disrupter, nonylphenol, using a microreactor composed of two reaction vessels stacked vertically through a microfluid filter. The filters constructed by deep X-ray lithography possessed 2100 through-bores (ϕ40×200 μm) in polymethylmethacrylate sheets (ϕ3 mm), which are appropriate for biochemical reactions. Through the optimization of the immunoassay, nonylphenol was quantitatively detected at the range of 0.1–10 ng/ml.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Katsuhiro Matsui, Isao Kawaji, Yuichi Utsumi, Yoshiaki Ukita, Toshifumi Asano, Masahiro Takeo, Dai-ichiro Kato, Seiji Negoro,