| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 23835 | Journal of Biotechnology | 2011 | 5 Pages |
Abstract
This study aimed to investigate precision of a newly developed gold nanoparticle-based immunoassay for measuring urinary N-telopeptides (NTx) and its correlation with ELISA measurement. NTx concentrations were determined by absorption changes using spectroscopy at 535 nm, which were measured three times with an interval of two hours for precision test, using urine samples of 60 subjects (32 males and 28 females; mean age 30.4 years, SD 21.9 years). The correlation of the immunoassay with the measurement of ELISA kit was also analyzed. Precision of the immunoassay and its correlation with ELISA were 0.924 (ICC, 95% confidence interval, 0.661-0.971) and 0.90 (p < 0.001). Our findings suggested that this immunoassay of urinary NTx could be used as a means of screening for bone resorption and point-of-care testing.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Min-Ho Lee, Chin Youb Chung, Kyoung Min Lee, Woo Kyeong Seong, Kyung shik Kim, Sang Dae Lee, Moon Seok Park,