In vitro Evaluation of Mesoporous Carbonated Hydroxyapatite in MC3T3-E1 Osteoblast Cells

Nanoporous bioceramic recently has gained attention as a drug storage and release host in the therapeutic application. Mesoporous carbonated hydroxyapatite (MCHA) is a nanoporous bioceramic with pore sized ranged between 2-50 nm. In order to use in drug delivery application, this material should demonstrate acceptable cytotoxic activity and osteoinductive response. Therefore, this study aimed to evaluate the effect of mesoporous carbonated hydroxyapatite on viability and alkaline phosphatase (ALP) activity of MC3T3-E1 osteoblasts cell line. These parameters represent cytotoxicity level and osteinductivity capability of the materials. MC3T3-E1 cells were cultured in 25 mg/ml extraction of mesoporous carbonated hydroxyapatite (meso-CHA) and non-porous CHA (np-CHA) for up to 7 days for viability and 14 days for ALP test. Results indicate that meso-CHA shows better cytotoxicity properties compare to np-CHA. Intracellular ALP of cells treated with meso-CHA was higher than np-CHA.