Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
23989 | Journal of Biotechnology | 2010 | 5 Pages |
Abstract
Transfection arrays are useful to analyze multiple genes at one time. In order to carry out gene transfection, cells are cultured on a plate on which genes are spotted to make extracellular matrix. However, this method is limited by low cell adhesion and transfection efficiency. To overcome these problems, we attempted to construct a novel extracellular matrix protein consisting of a variety of functional peptides. Here we fused the elastin derived peptide Ala-Pro-Gly-Val-Gly-Val (APGVGV) with the cell adhesive peptides, Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD). The resulting fusion proteins, E12PSGR, had high cell adhesive activity, transfection efficiency, and thermal stability.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
JooYoun Bae, Sayaka Goto, Masayasu Mie, Eiry Kobatake,