Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2402142 | Tuberculosis | 2006 | 4 Pages |
Abstract
Transposon mutagenesis produces random mycobacterial mutants at high frequency. Because they are random, however, it is difficult to isolate mutations in particular target genes. Here we describe the use of an arrayed library of Mycobacterium tuberculosis together with a PCR screening strategy to rapidly identify strains with defined insertion mutations. This method is useful for many genetic applications.
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Applied Microbiology and Biotechnology
Authors
James M. Lane, Eric J. Rubin,