Isolation of Thermophilic Microbes Resistant to Ethanol from Ciater Hot Springs, Subang-West Java

A study has been conducted to isolate thermophilic microbes from Ciater hot springs. The aim of this research is to select thermophilic microbes that resistant to ethanol. The methods of this research were: i. sampling, ii. enrichment, isolation and screening, iii. identification, and iv. determination of minimum inhibition concentration of ethanol. Enrichment, isolation and screening of thermophilic microbes done by using Luria Broth (LB) medium contained 4% of ethanol. Identification of isolates until genus level was done by comparing the results with those of other studies. Determination of Minimum Inhibitory Concentration (MIC) of ethanol was done by growing the isolates in the medium with different concentrations of ethanol (0%, 1%, 2%, 3%, 4%, 5%, 6%, 7% and 8%) and incubated at 50oC, for 24 h. The result of this study was obtained 6 thermophilic isolates which able to grow on a medium containing 4% ethanol (C1, C2, C3, C4, C5 and C6). From the results of MIC known that generally all isolates able to grow well until the ethanol content of 6%, except for C6 isolate which only grow maximum in 5% ethanol content. It was suspected that the bacteria isolated is a genus Thermoanaerobacter.