| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 24042 | Journal of Biotechnology | 2011 | 5 Pages |
Abstract
Escherichia coli endoribonuclease RNase E (Rne) regulates replication of ColE1-type plasmids by cleaving RNA I transcripts, which are synthesized from the plasmid and regulate the plasmid replication as antisense RNA. Here, we report the development of a genetic system that efficiently overproduces ColE1-type plasmid DNA when an RNase E variant that confers a hyperactive phenotype in RNA I cleavage is conditionally expressed from chromosome. This genetic system offers a method for isolation of large quantities of pure ColE1-type plasmid DNA, which have been most commonly used as molecular biology and biotechnology tools for research and industrial purposes.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Hayoung Go, Kangseok Lee,