Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
240601 | Procedia Chemistry | 2010 | 4 Pages |
High-performance liquid chromatographic methods were developed for the separation of the enantiomers of 19 β-lactams. The direct separations were performed on chiral stationary phases containing either amylose tris(3,5-dimethylphenylcarbamate), (Kromasil® AmyCoat™ column) or cellulose tris(3,5-dimethylphenylcarbamate), (Kromasil® CelluCoat™ column) as chiral selector. The different methods were compared in systematic chromatographic examinations. The separations were carried out with good selectivity and resolution. The AmyCoat™ and CelluCoat™ columns appear to be highly complementary. The best separations of bi- and tricyclic β-lactam stereoisomers were obtained with the AmyCoat™ column, while the 4-aryl-substituted β-lactams were better separated on the CelluCoat™ column. The elution sequence was determined in all cases; no general rule could be established.