Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
24251 | Journal of Biotechnology | 2009 | 4 Pages |
Abstract
High stability of the oligonucleotides immobilized on the glass is essential for the reliable DNA microarray analysis. In the present study, effect of end-capping of the unreacted silanol, remaining after the surface amine-functionalization, was explored: (1) Cy3-NHS (N-hydorxysuccinincimide) dye was spotted on the surface and change in the fluorescent intensity was measured. (2) DNA probes were immobilized by the reactivity of oxanine linked at the 5’-end, the complementary oligonucleotides with Cy5-fluorescence at the 5’-end was hybridized, and the time-dependence of the fluorescence intensity was observed. Both the systems showed improved stability of the immobilized molecules, indicative of the stabilization by end-capping.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Nagendra Kumar Kamisetty, Seung Pil Pack, Mitsuru Nonogawa, Kazunari Yamada, Yasuko Yoshida, Tsutomu Kodaki, Keisuke Makino,