Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
24558 | Journal of Biotechnology | 2008 | 6 Pages |
Abstract
A new approach to sequencing and assembling a highly heterozygous genome, that of grape, species Vitis vinifera cv Pinot Noir, is described. The combining of genome shotgun of paired reads produced by Sanger sequencing and sequencing by synthesis of unpaired reads was shown to be an efficient procedure for decoding a complex genome. About 2 million SNPs and more than a million heterozygous gaps have been identified in the 500 Mb genome of grape. More than 91% of the sequence assembled into 58,611 contigs is now anchored to the 19 linkage groups of V. vinifera.
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Bioengineering
Authors
Andrey Zharkikh, Michela Troggio, Dmitry Pruss, Alessandro Cestaro, Glenn Eldrdge, Massimo Pindo, Jeff T. Mitchell, Silvia Vezzulli, Satish Bhatnagar, Paolo Fontana, Roberto Viola, Alexander Gutin, Francesco Salamini, Mark Skolnick, Riccardo Velasco,