Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
24582 | Journal of Biotechnology | 2009 | 9 Pages |
Abstract
Glutamate dehydrogenase is a central enzyme connecting nitrogen and carbon metabolism via its precursors ammonium and oxoglutarate and its product glutamate. In Corynebacterium glutamicum glutamate dehydrogenase is especially important, since it is a key enzyme for the biotechnological production of the flavour enhancer l-glutamate. In this study, the regulation of gdh transcription was investigated. While originally described as a constitutively expressed gene, we could show that gdh transcription is highly variable depending on environmental conditions. Regulation of the gdh gene by the C. glutamicum nitrogen control protein AmtR was analyzed in detail in this study.
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Eva Hänßler, Tim Müller, Katrin Palumbo, Mirek Patek, Melanie Brocker, Reinhard Krämer, Andreas Burkovski,