Article ID Journal Published Year Pages File Type
2481768 European Journal of Pharmaceutical Sciences 2007 10 Pages PDF
Abstract

Doxorubicin-loaded long-circulating liposomes (Doxil™, ALZA Corp.) were additionally modified with the nucleosome-specific monoclonal antibody 2C5 (mAb 2C5) recognizing a broad variety of tumor cells via the tumor cell surface-bound nucleosomes. These mAb 2C5-modified PEGylated liposomes demonstrated 3–8-fold increase in the in vitro binding and internalization by multiple cancer cell lines of diverse origins (murine LLC, 4T1, C26 and human BT-20, MCF-7, and PC3), as shown by flow cytometry (FACS) and epi and confocal microscopy. As a result, mAb 2C5-modified Doxil™ demonstrated significantly higher cytotoxicity towards various cancer cells, including those resistant to doxorubicin, than all control preparations. The specific internalization of the mAb 2C5-Doxil™ into cytosol, along with the nuclear localization of their drug load, inside the target cancer cells were mainly responsible the superior anticancer activity. The IC50 values of mAb 2C5-Doxil™ with various murine and human cancer cells were 5–8-fold lower than those of control doxorubicin-loaded liposomes, Doxil™ or Doxil™ modified with a nonspecific IgG.

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