Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
24851 | Journal of Biotechnology | 2008 | 5 Pages |
Abstract
A synthetic medium, TK-25, for high cell density cultivation (HCDC) of Escherichia coli K-12 was modified to support HCDC of strain JM109. By optimizing the culture conditions, the cell concentration of 65 g/l in 14 h was obtained in the optimized medium, namely TK-10, with glucose-fed batch cultivation. When these conditions were further applied for HCDC of E. coli JM109 harboring pUC-based recombinant plasmid which expresses a hirudin variant, HV-1-fused protein under the control of trp promoter, it grew to 24 g/l of dried cells expressed as an inclusion body as 15.9% of the total protein, corresponding to 1908 mg/l hirudin-fused protein.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Toru Matsui, Hiroaki Sato, Hotaka Yamamuro, Satoru Misawa, Naoya Shinzato, Hitoshi Matsuda, Joji Takahashi, Seigo Sato,