Article ID Journal Published Year Pages File Type
2485880 Journal of Pharmaceutical Sciences 2007 13 Pages PDF
Abstract
The purpose of this investigation was to study the influences of absorption enhancers in increasing oral bioavailability of Ganciclovir (GAN) by assessing the transepithelial permeation across cell monolayers in vitro and bioavailability in rats in vivo. The permeation of GAN across Caco‐2 and MDCK cell monolayers in the absence/presence of dimethyl‐β‐cyclodextrin (DMβCD), chitosan hydrochloride (CH), sodium lauryl sulphate (SLS), and their combinations was studied for a 2‐h period. GAN was administered to rats in absence/presence of absorption enhancers and drug contents in plasma were estimated. We found that the apparent permeability coefficient (Papp) of GAN in absence of absorption enhancers (control) were 0.261 ± 0.072 × 10−6 and 0.486 ± 0.063 × 10−6 cm/s in Caco‐2 and MDCK cell monolayers, respectively, whereas in the presence of DMβCD, CH, SLS, and their combinations, Papp of GAN increased by 5‐ to 25‐fold and 7‐ to 33‐fold as compared to control in Caco‐2 and MDCK cell monolayers, respectively. However, in rats, the maximum enhancement in bioavailability of GAN during coadministration of these absorption enhancers was only fivefold compared to GAN control. To conclude, the absorption enhancers‐DMβCD, CH, SLS, and their combinations demonstrated significant improvement in transepithelial permeation and bioavailability of GAN. © 2007 Wiley‐Liss, Inc. and the American Pharmacists Association J Pharm Sci 96: 2710-2722, 2007
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Health Sciences Pharmacology, Toxicology and Pharmaceutical Science Drug Discovery
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