| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2485886 | Journal of Pharmaceutical Sciences | 2007 | 7 Pages |
Abstract
This study describes a fast spectrophotometic titration method for apparent ionization constant (pKa) determination. In this method, a Universal pH indicator is utilized instead of the conventional pH electrode. An autoburette is set to add HCl at a constant rate to a vigorously stirred 1 cm UV cuvette which contains sample and indicator solution. A spectrophotometer continuously records the spectra. Acquired spectral data are processed by calculating the pH from the indicator spectra in the visible region and extracting sample spectra from the UV region. Five compounds possessing pKa values in the range 2-10 were investigated. These results differed from measurements by conventional spectrophotometric titration by ±0.05 to ±0.10 log unitand the American Pharmacists Association J Pharm Sci 96: 2777-2783, 2007
Related Topics
Health Sciences
Pharmacology, Toxicology and Pharmaceutical Science
Drug Discovery
Authors
Xiaole Kong, Tao Zhou, Zudong Liu, Robert C. Hider,