Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2486026 | Journal of Pharmaceutical Sciences | 2010 | 8 Pages |
Abstract
N-terminus-specific PEGylation was used to produce mono-PEGylated lysozyme. However, some di- and tri-PEGylated proteins were also produced due to side chain reaction. The reaction products were characterized by chromatographic and electrophoretic methods. Commercial cation exchange membrane Sartobind S was used for chromatographic purification of PEGylated lysozyme, the basis of separation being the shielding of protein charge by PEG. Using the membrane chromatographic method, lysozyme and mono-, di-, and tri-PEGylated lysozyme could be resolved into separate peaks. Increasing the superficial velocity during chromatographic separation from 24Â cm/h to 240Â cm/h increased both protein binding capacity and resolution due to enhancement of protein mass transfer coefficient.
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Authors
Deqiang Yu, Raja Ghosh,