Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2486350 | Journal of Pharmaceutical Sciences | 2011 | 12 Pages |
Abstract
The objective of this study is to engineer polylysine-heparin functionalized solid lipid nanoparticles (fSLNs) for the use of a vaginal microbicide delivery template for HIV prevention. The fSLNs are prepared using a modified phaseâinversion technique followed by a layerâbyâlayer deposition method. The Box-Behnken experimental design is used to analyze the influence of three factors (X1 = bovine serum albumin concentration, X2 = pH of the aqueous phase, and X3 = lipid amount) on the particle mean diameter (PMD) measured by dynamic light scattering (DLS). Tenofovir is used as a model antiâHIV microbicide. The SLNs are also characterized for morphology, zeta potential (ζ ), percent drug encapsulation efficiency (EE%), and cytotoxicity on a human vaginal epithelial cell line by electron microscopy, DLS, ultraviolet, and fluorescence spectroscopy, respectively. The statistical model predicts particle size (Y) with 90% confidence and the Y values are significantly affected by X1 and X2. The produced fSLNs appear noncytotoxic and exhibit a plateletâlike shape with respective PMD, EE%, and ζ value of 153 nm, 8.3%, and â 51mV. These fSLNs intended to be administered topically have the potential to enhance cellular uptake of hydrophobic microbicides and outdistance the virus during the HIV/AIDS infection process, possibly leading to more effective prevention of the disease transmission. © 2011 WileyâLiss, Inc. and the American Pharmacists Association J Pharm Sci 100:3345-3356, 2011
Keywords
Related Topics
Health Sciences
Pharmacology, Toxicology and Pharmaceutical Science
Drug Discovery
Authors
Dima Alukda, Timothy Sturgis, BiâBotti C. Youan,