Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2486670 | Journal of Pharmaceutical Sciences | 2011 | 9 Pages |
Abstract
Oil-frozen water-in-oil-in-water (W1/O/W2) double emulsions (DE) containing ethanol up to 40% (w/v) in the external aqueous W2 phase exhibited external coalescence upon thawing of the oil phase, releasing up to 85% of the encapsulated protein of the internal aqueous phase. These emulsions were studied in vitro as potential dermal macromolecular delivery formulations, achieving fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) penetration of up to 86 μm into porcine skin, reaching the viable epidermis where the immunocompetent Langerhans cells are located. Enzyme-linked immunosorbent assay was performed to observe the effect of the emulsification process and ethanol content on the ability of BSA to form antigen-antibody complexes; results indicated that ethanol content and the emulsification process did not diminish the BSA-antibody complex formation when compared with a BSA standard aqueous solution. Therefore, it is shown that oil-frozen W1/O/W2 DE, with penetration-enhancing ethanol in the W2 phase, can potentially be used for cutaneous vaccine delivery formulations.
Keywords
Related Topics
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Pharmacology, Toxicology and Pharmaceutical Science
Drug Discovery
Authors
Yuly A. Jaimes-Lizcano, Louise B. Lawson, Kyriakos D. Papadopoulos,