Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2487327 | Journal of Pharmaceutical Sciences | 2008 | 9 Pages |
Abstract
The aim of this study is to prepare cisplatinâincorporated nanoparticles based on ion complex formation between hyaluronic acid (HA) and cisplatin for antitumor drug delivery. To prepare nanoparticles using HA, bulk HA was degraded by hyaluronidases (HAses). Cisplatinâincorporated HA nanoparticles were prepared by mixing cisplatin with an aqueous solution of HA and then the nanoparticle solution was dialyzed to remove trace elements. Since glioma tumor cell lines are able to secrete HAse, extracts from U343MG and U87MG cell lines were used to test the release of cisplatin from the nanoparticles. The morphological observation of the cisplatinâincorporated nanoparticles showed that they had spherical shapes with a particle size around 100-200 nm. The loading efficiency of cisplatin in the nanoparticles was about 67-81% (w/w) and cisplatin was continuously released from the nanoparticles for 4 days. Especially, the release rate of cisplatin from the nanoparticles increased when HAse was added to the release medium. In the results of the HA zymography, the U343MG cell line secreted HAse, while the U87MG cell line did not. When the extracts from U343MG were added to the release medium, the release rate of cisplatin was slightly increased, while the extracts from U87MG did not significantly affect the release rate of cisplatin. In conclusion, cisplatinâincorporated nanoparticles have sufficiently small particle sizes to use as a drug targeting system. The release of cisplatin from the nanoparticles was responsive to the secretion of HAse. These nanoparticles are suitable vehicles for an antitumor drug targeting system. © 2007 WileyâLiss, Inc. and the American Pharmacists Association J Pharm Sci 97:1268-1276, 2008
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Authors
YoungâIl Jeong, SeongâTaek Kim, ShuâGuang Jin, HyangâHwa Ryu, YongâHao Jin, TaeâYoung Jung, InâYoung Kim, Shin Jung,