| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2488719 | Journal of Young Pharmacists | 2012 | 7 Pages |
Abstract
A rapid, simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for quantification of olmesartan medoxomil (OLM) and amlodipine besylate (AM) in plasma. The assay enables the measurement of OLM and AM for therapeutic drug monitoring with a minimum detectable limit of 2 ng mL. The method involves a simple, one-step extraction procedure and analytical recovery was above 50%. The Separation was performed on an analytical 250 * 4.6 mm Eurospher 100-Â 5 C18 column. The wavelength was set at239 nm. The mobile phasewas a mixture of acetonitrile:0.05 M ammoniumacetate buffer: 0.1 mLtriethylamine at pH 6.8 was selected at a flow rate of 1.0 mL/min. The calibration curve for the determination of OLM and AM in plasma was linear overthe ränge 2-2500 and 8-10,000 ng/mLAM and OLM. The coefficients of Variation for interday and intraday assay were found to be <Â 15%. The method can be applied to a pharmacokinetic and pharmacodynamic study of OLM and AM in a combined dosage form.
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Authors
S.K. Shah, A.J. Asnani, D.P. Kawade, S.C. Dangre, S.K. Arora, Yende S.R.,