Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
24924 | Journal of Biotechnology | 2008 | 6 Pages |
Abstract
We investigated the direct constitution of membrane proteins into giant liposomes in cell-free (in vitro) protein synthesis. Giant liposomes were present in a translation reaction cocktail of a wheat germ cell-free protein translation system. Apo cytochrome b5 (b5) and its fusion proteins were synthesized and directly localized in the liposomes. After the translation reaction, the proteo-liposomes were isolated by simplified discontinuous density-gradient centrifugation. Apo cytochrome b5 conjugated dihydrofolate reductase (DHFR) was synthesized in the same procedure and the protein was directly displayed on the liposome surface. b5 acts as a “hydrophobic tag” for recruitment to the liposome surface.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Shin-ichiro M. Nomura, Satoshi Kondoh, Wakiko Asayama, Akikazu Asada, Shigemichi. Nishikawa, Kazunari Akiyoshi,