| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2508053 | Journal of Pharmaceutical Analysis | 2012 | 7 Pages |
Abstract
This paper sets out to summarize the literatures based on immobilized enzyme bio-chromatography and its application in inhibitors screening in the last decade. In order to screen enzyme inhibitors from a mass of compounds in preliminary screening, multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes, and then the immobilized enzyme reactor applied as the immobilized enzyme stationary phase in HPLC. Therefore, a technology platform of high throughput screening is gradually established to screen the enzyme inhibitors as new anti-tumor drugs. Here, we briefly summarize the selective methods of supports, immobilization techniques, co-immobilized enzymes system and the screening model.
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Authors
Si-Meng Fang, Hai-Na Wang, Zhong-Xi Zhao, Wei-Hong Wang,