Article ID Journal Published Year Pages File Type
25238 Journal of Biotechnology 2006 10 Pages PDF
Abstract

The submerged culture of the entomopathogenic nematode Steinernema carpocapsae and its symbiotic bacterium, Xenorhabdus nematophila, was carried out in orbitally agitated bottles using a culture medium containing whey (in grams per litre: 500 whey, 20 yeast extract, 10 dried egg yolk-food grade, 3 sodium chloride, 37 corn oil-food grade). Maximum total viable nematode concentrations of 198,333 ml−1 were achieved within fermentations of 24 days with 64% of the nematode population within the infective juvenile stage (IJ) (126,666 ml−1) at the end. The kinetics of the bioprocess was well modelled using the four-parameter Sigmoidal model and the corresponding maximum specific rates of nematode production (0.47 day−1), carbohydrates consumption (0.0008 gcarbohydrates gnematodes−1 day−1) and nitrogen consumption (4.44 gnitrogen gnematodes−1 day−1) are first proposed. Besides, X. nematophila appears to have the capacity of lactose hydrolysis.

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Physical Sciences and Engineering Chemical Engineering Bioengineering
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