| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2538755 | Fitoterapia | 2012 | 7 Pages |
This manuscript reports on the first CE-method suitable for analyzing twelve pharmacologically relevant compounds (flavonoids, caffeic acids and leontopodic acid) in the aerial parts of Leontopodium alpinum. By using a 60 mM borax buffer containing 25 mM SDS and 35% acetonitrile the analytes could be well resolved in 25 min and accurately determined in different Edelweiss specimens. Method validation assured the compliance of the assay to ICH standards (correlation coefficients ≥ 0.999, LOD ≤ 7.2 μg/ml, LOQ ≤ 23.0 μg/ml, recovery rates from 96.6 to 101.1%, intermediate precision ≤ 7.01%, and repeatability ≤ 5.52%). The quantitative results revealed a strong dependence of the content of phenolics on the growth status of the plants. In early stages no flavonoids were found, while during blooming all metabolites reached higher levels; commercially cultivated plant material showed the overall highest values (flavonoids: 0.25–0.34%, caffeic acids: 4.99–6.21%, leontopodic acid: 4.21–5.05%).
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