A new coumestan with immunosuppressive activities from Flemingia philippinensis

A new coumestan, 1,3,9-trihydroxy-8-methoxycoumestan, named flemicoumestan A 1 together with nine isoflavone-related compounds were isolated from the ethyl acetate extract of the roots of Flemingia philippinensis. Their structures were elucidated and confirmed by spectroscopic methods and literature data. Compounds 3 and 4 showed strong lymphocyte proliferation inhibitory activity with an IC50 value of 1.04–2.76 μM, and the low cytotoxicity with the CC50 value of 71.01 and 56.36 μM, respectively.

Graphical abstractA novel coumestan, flemicoumestan A (1), together with nine known compounds, was isolated from the ethyl acetate extract of the roots of Flemingia philippinensis. The structures were determined by spectroscopic methods. All the compounds were evaluated for their immunosuppressive activities in vitro.Figure optionsDownload full-size imageDownload as PowerPoint slide