Chemical constituents of Kigelia pinnata twigs and their GLUT4 translocation modulatory effect in skeletal muscle cells

Phytochemical investigation of the ethanolic extract of twigs of Kigelia pinnata DC. afforded one new iridoid 7-hydroxy eucommiol (1), and nine known compounds (2–10). The structure of compounds was elucidated by extensive spectroscopic methods, including 1D, 2D NMR experiments and MS analysis. All these compounds were evaluated for GLUT4 translocation modulatory effect in skeletal muscle cells. Four of the tested compounds 1, 5, 6 and 7 showed significant stimulation of GLUT4 translocation to cell surface in skeletal muscle cells without any adverse effect on cell viability. Effect of these four compounds was concentration-dependent and comparable to standard drug rosiglitazone. These findings indicate that constituents of K. pinnata may provide leads for the therapeutics for insulin resistance and diabetes.

Graphical abstractPhytochemical investigation of Kigelia pinnata DC. afforded one new (1), and nine known compounds (2–10). The structures of compounds were elucidated by extensive spectroscopic techniques. Compounds 1, 5, 6 and 7 showed significant stimulation of GLUT4 translocation to cell surface in skeletal muscle cells.Figure optionsDownload full-size imageDownload high-quality image ( K)Download as PowerPoint slideHighlights► One new compound along with nine known compounds was isolated from twig of Kigelia pinnata. ► All compounds were evaluated for GLUT4 translocation modulatory effect in skeletal muscle cells. ► Compounds 1, 5, 6 and 7 showed significant activity.