Molecular characterization and marker based chemotaxonomic studies of Podophyllum hexandrum Royle

Detailed chemical studies and RAPD analysis were done in different populations of Podophyllum hexandrum collected from high altitude regions of North Western Himalayas. Random amplified polymorphic DNA (RAPD) analysis revealed a high degree of genetic diversity among the 12 collected accessions, attributed to their geographical and climatic conditions. HPLC analysis also revealed variation in the concentration of two major marker compounds which lead to the identification of a chemotype. The study demonstrated that RAPD and chemical markers are very useful tools to compare the genetic relationship and pattern of variation among such prioritized and endangered medicinal plants.

Graphical abstractPodophyllum hexandrum contains two major lignans such as podophyllotoxin and podophyllotoxin-β-D-glycoside, which are the marker compounds that can differentiate different accessions as basis for identification of a chemotype.Figure optionsDownload full-size imageDownload as PowerPoint slide