| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 25394 | Journal of Biotechnology | 2007 | 4 Pages |
Abstract
Wild-type phenylalanine dehydrogenase from Bacillus sphaericus, and three mutants N145A, N145V and N145L, are used with a coenzyme recycling system to synthesise l-phenylalanine and three non-natural amino acids (p-F-phenylalanine, p-MeO-phenylalanine and p-CF3-phenylalanine) on a millimole scale. A range of reaction conditions are investigated. The kinetic parameters of WT PheDH and N145A towards p-CF3-phenylpyruvate are compared, emphasising the value of protein engineering in creating improved biocatalysts.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Francesca Paradisi, Stuart Collins, Anita R. Maguire, Paul C. Engel,