Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2539613 | Fitoterapia | 2009 | 4 Pages |
Abstract
Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.
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Authors
Osamu Morinaga, Takuhiro Uto, Seiichi Sakamoto, Hiroyuki Tanaka, Yukihiro Shoyama,