| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2544632 | Journal of Ethnopharmacology | 2016 | 7 Pages |
Ethno-pharmacological relevanceBu-Shen-Gu-Chi-Wan is a Chinese patent medicine for the treatment of chronic periodontitis. It has an anti-inflammatory function in experimental periodontitis and can improve alveolar bone remodeling.Aim of the studyThis study aims to explore the effects of Bu-Shen-Gu-Chi-Wan on serum metabolism in a rat model of periodontitis using 1H nuclear magnetic resonance (1H NMR) based metabonomics.Materials and methodsThe model of experimental periodontitis in a rat was established by steel wire ligation, plus a high glucose diet and Porphyromonas gingivalis inoculation. When the models had been established, 6-week-old Sprague-Dawley female rats (n=31) were divided into 5 groups: high dose group (Group H), medium dose group (Group M), low dose group (Group L), periodontitis group (Group P) and healthy control group (Group N). Rats in Group H, M and L were given the Bu-Shen-Gu-Chi-Wan solution (0.8, 2 and 4 g/kg of body weight) daily for 60 days. Rats in Group P and N were administered normal saline (10 ml/kg of body weight) in the same period. All rats were sacrificed at the end of the study and serum samples were collected. The metabolites in the serum were analyzed using 1H NMR in conjunction with principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA).ResultsThe metabonomic profiles of five groups showed the clustering of individual dataset in every group, and the 1st principal component and the 2nd principal component could significantly differentiate the datasets of medium dose or high dose group from other groups. The chemical shift regions at δ 1.22 ppm, 1.86 ppm, 2.26 ppm, 2.34 ppm and 2.42 ppm showed the most obvious differences among the five groups. The correspondent metabolites were high density lipoprotein, pyruvate/oxaloacetate, arginine and glutamine.ConclusionThe effects of Bu-Shen-Gu-Chi-Wan on the rat serum metabolites were dose dependent. High density lipoprotein, pyruvate/oxaloacetate, arginine and glutamine may be the serum biomarkers of Bu-Shen-Gu-Chi-Wan.
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