Evaluation of guggulipid and nimesulide on production of inflammatory mediators and GFAP expression in LPS stimulated rat astrocytoma, cell line (C6)

Aim of the studyThe present study was designed to investigate effect of guggulipid, a drug developed by CDRI and nimesulide on LPS stimulated neuroinflammatory changes in rat astrocytoma cell line (C6).Materials and methodsRat astrocytoma cells (C6) were stimulated with LPS (10 μg/ml) alone and in combinations with different concentrations of guggulipid or nimesulide for 24 h of incubation. Nitrite release in culture supernatant, ROS in cells, expressions of COX-2, GFAP and TNF-α in cell lysate were estimated.ResultsLPS (10 μg/ml) stimulated C6 cells to release nitrite, ROS generation, up regulated COX-2 and GFAP expressions at protein level and TNF-α at mRNA level. Both guggulipid and nimesulide significantly attenuated nitrite release, ROS generation and also down regulated expressions of COX-2, GFAP and TNF-α. Guggulipid and nimesulide per se did not have any significant effect on C6 cells.ConclusionResults demonstrate the anti-inflammatory effect of guggulipid comparable to nimesulide which suggest potential use of guggulipid in neuroinflammation associated conditions in CNS disorders.

Graphical abstractEffect of nimesulide and guggulipid on LPS induced neuronal cell death.Figure optionsDownload full-size imageDownload as PowerPoint slide