In vivo anti-inflammatory and antinociceptive actions of some Lamium species

Ethnopharmacological relevanceSeveral Lamium species have been used to relieve pain in arthritic ailments in Turkish folk medicine.Aim of the studyTo evaluate the anti-inflammatory and antinociceptive activities of different extracts prepared with methanol, dichloromethane, n-butanol and water from the aerial parts of some Lamium species of Turkish origin.Materials and methodsLamium eriocephalum subsp. eriocephalum, Lamium garganicum subsp. laevigatum, Lamium garganicum subsp. pulchrum and Lamium purpureum var. purpureum (Lamiaceae); for the anti-inflammatory activity, carrageenan-induced hind paw edema model, PGE2-induced hind paw edema model, and 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema model and for the antinociceptive activity p-benzoquinone (PBQ)-induced writhing test in mice were employed.ResultsThe n-butanol extracts of Lamium garganicum subsp. laevigatum (LGL-BuOH), Lamium garganicum subsp. pulchrum (LGP-BuOH), and Lamium purpureum var. purpureum (LPP-BuOH) exhibited notable inhibition (16.5–28.9, 14.5–26.9, 12.3–21.5%, resp.) in carrageenan-induced hind paw edema model at doses of 200 mg/kg without inducing any gastric damage. The LGL-BuOH (7.1–30.4%) and LGP-BuOH (5.9–24.1%) extracts also displayed potent anti-inflammatory activity against PGE2-induced hind paw edema model. LGL-BuOH and LGP-BuOH were also found to exhibit remarkable antinociceptive activity in p-benzoquinone-induced abdominal constriction test at a dose of 200 mg/kg (25.0 and 24.3%, respectively).ConclusionThe experimental data demonstrated that Lamium garganicum subsp. laevigatum and Lamium garganicum subsp. pulchrum displayed remarkable anti-inflammatory and antinociceptive activities.