Ondamtanggamibang protects neurons from oxidative stress with induction of heme oxygenase-1

Ondamtanggamibang (ODG) has been used as a remedy to treat psychological anxiety and depression in Oriental medicine. In this study, we found the protective effects of ODG against oxidative stress by induction of the antioxidative enzyme, heme oxygenase (HO)-1 in neuronal PC12 cells. Pretreatment with ODG extract protected neuronal cells from damage induced by H2O2 and 6-hydroxydopamine (6-OHDA), but simultaneous treatment with ODG extract did not. ODG also inhibited the intracellular reactive oxygen species elevation by H2O2 and 6-OHDA. ODG stimulation strongly induced the expression of HO-1 in PC12 cells. The protective effect of ODG extract on oxidative stress-induced damage was suppressed by HO inhibitor, zinc protoporphyrin IX (ZnPP-IX). Taken together, these data suggest that ODG treatment has potential protective effects in neuronal cells under oxidative stress.