Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2548623 | Journal of Ethnopharmacology | 2006 | 6 Pages |
The species of Clematis (Ranunculaceae) have been traditionally used for inflammatory conditions by indigenous Australians. We have previously reported that the ethanol extract of Clematis pickeringii inhibited COX-1. In this study, we examined the ethanol extracts and fractions of three Clematis species, Clematis pickeringii, Clematis glycinoides and Clematis microphylla, on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). We further examined the activating effects on the protein expression of peroxisome proliferator-activated receptor alpha (PPARα) and gamma (PPARγ) in HepG2 cells. The ethanol extracts of three Clematis species inhibited the activities of COX-1, COX-2 and 5-LOX in the different extents. The stem extract of Clematis pickeringii showed the highest inhibitory activities among the three species on COX-1, COX-2 and 5-LOX with the IC50 values of 73.5, 101.2 and 29.3 μg/mL. One of its fractions also significantly elevated PPARα expression by 173, 280 and 435% and PPARγ expression by 140, 228 and 296% at 4, 8 and 16 μg/mL, respectively.