Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2549032 | Journal of Pharmacological and Toxicological Methods | 2015 | 6 Pages |
Abstract
The novel custom LDH release assay allows the reproducible quantification of cell viability and is highly cost-effective when compared to commercially available assays (approximately 25 times cheaper). In addition and in contrast to commercially available assays, the identification and detailed description of all assay components and procedures provide greater control over experimental conditions and design. We provide a detailed standard operating procedure permitting our novel assay to be readily adapted depending on experimental requirements.
Keywords
PBSintHEPESMPMstBHPtert-butylhydroperoxideDCFDAEtOH3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acidMTTROSEthanolanalysis of varianceANOVATroloxPlate readerCell viabilityC6 glioma cellhigh-throughput screeninglactate dehydrogenaseLDHPhosphate-buffered salineNADReactive oxygen speciesGliaiodonitrotetrazolium chloride
Related Topics
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Authors
Simon Kaja, Andrew J. Payne, Tulsi Singh, Jasleen K. Ghuman, Erin G. Sieck, Peter Koulen,