Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
25540 | Journal of Biotechnology | 2007 | 12 Pages |
A regulative two-component system previously identified in Pseudoalteromonas haloplanktis TAC125 was used to construct a cold inducible expression system that is under the control of l-malate. Performances of the inducible system were tested for both psychrophilic and mesophilic protein production using two “difficult” proteins as control. The results obtained demonstrated that both psychrophilic β-galactosidase and yeast α-glucosidase are produced in a fully soluble and catalytically competent form. Optimal conditions for protein production, including growth temperature, growth medium and l-malate concentration were also investigated. Under optimized conditions yields of 620 and 27 mg/l were obtained for β-galactosidase and α-glucosidase, respectively.