Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
25603 | Journal of Biotechnology | 2006 | 7 Pages |
Abstract
Environmental DNA libraries are important sources for novel biocatalyst genes but activity screening for relevant enzymes is often inefficient. Therefore, we have constructed the transposon MuExpress which randomly integrates in vitro into existing bacterial artificial chromosome (BAC) or cosmid libraries and permits the inducible expression of its flanking regions in both directions. Furthermore, this transposon allows the bidirectional sequencing of the respective clones starting from unique primer binding sites.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Christian Leggewie, Helge Henning, Christel Schmeisser, Wolfgang R. Streit, Karl-Erich Jaeger,